Human Pathology
Volume 37, Issue 3 , Pages 361-368 , March 2006

Contribution of microdissection for the detection of microsatellite instability in colorectal cancer

  • Marie Danjoux

      Affiliations

    • INSERM U563, Centre de Physiopathologie de Toulouse Purpan, CHU Purpan, 31059 Toulouse, France
    • Service d'Anatomie et de Cytologie Pathologiques, CHU Purpan, 31059 Toulouse, France
    • ,
  • Rosine Guimbaud

      Affiliations

    • INSERM U563, Centre de Physiopathologie de Toulouse Purpan, CHU Purpan, 31059 Toulouse, France
    • Département d'oncologie médicale, Centre Claudius Regaud, 20-24, rue du pont St Pierre, 31052 Toulouse, France
    • ,
  • Talal Al Saati

      Affiliations

    • Plate-forme d'histopathologie expérimentale, IFR30, CHU Purpan, 31059 Toulouse, France
    • ,
  • Fabienne Meggetto

      Affiliations

    • INSERM U563, Centre de Physiopathologie de Toulouse Purpan, CHU Purpan, 31059 Toulouse, France
    • ,
  • Nicolas Carrère

      Affiliations

    • Services de chirurgie digestive, CHU Purpan, 31059 Toulouse, France
    • ,
  • Guillaume Portier

      Affiliations

    • Services de chirurgie digestive, CHU Purpan, 31059 Toulouse, France
    • ,
  • Georges Delsol

      Affiliations

    • INSERM U563, Centre de Physiopathologie de Toulouse Purpan, CHU Purpan, 31059 Toulouse, France
    • ,
  • Janick Selves

      Affiliations

    • INSERM U563, Centre de Physiopathologie de Toulouse Purpan, CHU Purpan, 31059 Toulouse, France
    • Service d'Anatomie et de Cytologie Pathologiques, CHU Purpan, 31059 Toulouse, France
    • Corresponding Author InformationCorresponding author. Laboratoire D'Anatomie Pathologique, CHU-Purpan Place du Docteur Baylac, 31059 Toulouse Cedex, France.

Received 1 January 2005 ,Accepted 1 June 2005.

References 

  1. Kinzler KW, Vogelstein B. Lessons from hereditary colorectal cancer. Cell. 1996;87:159–170
  2. Ionov Y, Peinado MA, Malkhosyan S, Shibata D, Perucho M. Ubiquitous somatic mutations in simple repeated sequences reveal a new mechanism for colonic carcinogenesis. Nature. 1993;363:558–561
  3. Thibodeau SN, Bren G, Schaid D. Microsatellite instability in cancer of the proximal colon. Science. 1993;260:816–819
  4. Aaltonen LA, Peltomaki P. Genes involved in hereditary nonpolyposis colorectal carcinoma. Anticancer Res. 1994;14:1657–1660
  5. Aaltonen LA, Peltomaki P, Leach FS, et al. Clues to the pathogenesis of familial colorectal cancer. Science. 1993;260:812–816
  6. Tannergard P, Liu T, Weger A, Nordenskjold M, Lindblom A. Tumorigenesis in colorectal tumors from patients with hereditary non-polyposis colorectal cancer. Hum Genet. 1997;101:51–55
  7. Gafa R, Maestri I, Matteuzzi M, et al. Sporadic colorectal adenocarcinomas with high-frequency microsatellite instability. Cancer. 2000;89:2025–2037
  8. Cunningham JM, Christensen ER, Tester DJ, et al. Hypermethylation of the hMLH1 promoter in colon cancer with microsatellite instability. Cancer Res. 1998;58:3455–3460
  9. Elsaleh H, Joseph D, Grieu F, Zeps N, Spry N, Iacopetta B. Association of tumour site and sex with survival benefit from adjuvant chemotherapy in colorectal cancer. Lancet. 2000;355:1745–1750
  10. Gryfe R, Kim H, Hsieh ET, et al. Tumor microsatellite instability and clinical outcome in young patients with colorectal cancer. N Engl J Med. 2000;342:69–77
  11. Hemminki A, Mecklin JP, Jarvinen H, Aaltonen LA, Joensuu H. Microsatellite instability is a favorable prognostic indicator in patients with colorectal cancer receiving chemotherapy. Gastroenterology. 2000;119:921–928
  12. Wright CM, Dent OF, Barker M, et al. Prognostic significance of extensive microsatellite instability in sporadic clinicopathological stage C colorectal cancer. Br J Surg. 2000;87:1197–1202
  13. Wild P, Knuechel R, Dietmaier W, Hofstaedter F, Hartmann A. Laser microdissection and microsatellite analyses of breast cancer reveal a high degree of tumor heterogeneity. Pathobiology. 2000;68:180–190
  14. Bohm M, Wieland I, Stinhofer C, Otto T, Rubben H. Detection of loss of heterozygosity in the APC tumor suppressor gene in nonpapillary renal cell carcinoma by microdissection and polymerase chain reaction. Urol Res. 1997;25:161–165
  15. Baisse B, Bouzourene H, Saraga EP, Bosman FT, Benhattar J. Intratumor genetic heterogeneity in advanced human colorectal adenocarcinoma. Int J Cancer. 2001;93:346–352
  16. Gravel S, Delsol G, Al Saati T. Single-cell analysis of the t(14;18)(q32;q21) chromosomal translocation in Hodgkin's disease demonstrates the absence of this translocation in neoplastic Hodgkin and Reed-Sternberg cells. Blood. 1998;91:2866–2874
  17. Dietmaier W, Wallinger S, Bocker T, Kullman F, Fischel R, Ruschoff J. Diagnostic microsatellite instability: definition and correlation with mismatch repair protein expression. Cancer Res. 1997;57:4749–4756
  18. Bocker T, Diermann J, Friedl W, et al. Microsatellite instability analysis: a multicenter study for reliability and quality control. Cancer Res. 1997;57:4739–4743
  19. Boland CR, Thibodeau SN, Hamilton SR, et al. A National Cancer Institute Workshop on Microsatellite Instability for cancer detection and familial predisposition: development of international criteria for the determination of microsatellite instability in colorectal cancer. Cancer Res. 1998;58:5248–5257
  20. Suraweera N, Duval A, Reperant M, et al. Evaluation of tumor microsatellite instability using five quasimonomorphic repeats and pentaplex PCR. Gastroenterology. 2002;123:1804–1811
  21. Zhou XP, Hoang JM, Cottu P, Thomas G, Hamelin R. Allelic profiles of mononucleotide repeat microsatellites in control individuals and in colorectal tumors with and without replication errors. Oncogene. 1997;15:1713–1718
  22. Zhou XP, Hoang JM, Li YJ, et al. Determination of the replication error phenotype in human tumors without the requirement for matching normal DNA by analysis of mononucleotide repeat microsatellites. Genes Chromosomes Cancer. 1998;21:101–107
  23. Dietmaier W, Gansbauer S, Beyser K, et al. Microsatellite instability in tumor and nonneoplastic colorectal cells from hereditary non-polyposis colorectal cancer and sporadic high microsatellite-instable tumor patients. Pathobiology. 2000;68:227–231
  24. Shibata D, Peinado MA, Ionov Y, Malkhosyan S, Perucho M. Genomic instability in repeated sequences is an early somatic event in colorectal tumorigenesis that persists after transformation. Nat Genet. 1994;6:273–281
  25. Samowitz WS, Slattery ML. Regional reproducibility of microsatellite instability in sporadic colorectal cancer. Genes Chromosomes Cancer. 1999;26:106–114
  26. Barnetson R, Jass J, Tse R, Eckstein R, Robinson B, Schnitzler M. Mutations associated with microsatellite unstable colorectal carcinomas exhibit widespread intratumoral heterogeneity. Genes Chromosomes Cancer. 2000;29:130–136
  27. Bertheau P, Plassa LF, Lerebours F, et al. Allelic loss detection in inflammatory breast cancer: improvement with laser microdissection. Lab Invest. 2001;81:1397–1402
  28. Charafe-Jauffret E, Moulin JF, Ginestier C, et al. Loss of heterozygosity at microsatellite markers from region p11-21 of chromosome 8 in microdissected breast tumor but not in peritumoral cells. Int J Oncol. 2002;21:989–996
  29. Stoehr R, Wild P, Hartmann A. Lasermicrodissection—an important prerequisite for the molecular-genetic analysis of bladder cancer. Pathol Res Pract. 2003;199:355–362
  30. Giercksky HE, Thorstensen L, Qvist H, Nesland JM, Lothe RA. Comparison of genetic changes in frozen biopsies and microdissected archival material from the same colorectal liver metastases. Diagn Mol Pathol. 1997;6:318–325
  31. Goel A, Arnold CN, Niedzwiecki D, et al. Characterization of sporadic colon cancer by patterns of genomic instability. Cancer Res. 2003;63:1608–1614
  32. Khine K, Smith DR, Goh HS. High frequency of allelic deletion on chromosome 17p in advanced colorectal cancer. Cancer. 1994;73:28–35
  33. Ogunbiyi OA, Goodfellow PJ, Herfarth K, et al. Confirmation that chromosome 18q allelic loss in colon cancer is a prognostic indicator. J Clin Oncol. 1998;16:427–433

☆ This work was supported by a grant from the “Projet Hospitalier de Recherche Clinique” (no. 00-08) and the “Ligue contre le cancer.” We acknowledge the “Délégation Régionale à la Recherche Clinique des Hopitaux de Toulouse” (AOL no. 98-36L) for its financial support.

PII: S0046-8177(05)00753-7

doi: 10.1016/j.humpath.2005.06.022

Human Pathology
Volume 37, Issue 3 , Pages 361-368 , March 2006

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