Human Pathology
Volume 40, Issue 7 , Pages 924-933 , July 2009

Specific detection of prostate cancer cells in urine by multiplex immunofluorescence cytology

  • Kazutoshi Fujita, MD

      Affiliations

    • Department of Pathology, The Johns Hopkins Medical Institutions, Baltimore, MD 21231, USA
    • Department of Urology, The Johns Hopkins Medical Institutions, Baltimore, MD 21231, USA
    • ,
  • Christian P. Pavlovich, MD

      Affiliations

    • Department of Urology, The Johns Hopkins Medical Institutions, Baltimore, MD 21231, USA
    • ,
  • George J. Netto, MD

      Affiliations

    • Department of Pathology, The Johns Hopkins Medical Institutions, Baltimore, MD 21231, USA
    • Department of Urology, The Johns Hopkins Medical Institutions, Baltimore, MD 21231, USA
    • Department of Oncology, The Johns Hopkins Medical Institutions, Baltimore, MD 21231, USA
    • ,
  • Yuko Konishi, BS

      Affiliations

    • Department of Pathology, The Johns Hopkins Medical Institutions, Baltimore, MD 21231, USA
    • ,
  • William B. Isaacs, PhD

      Affiliations

    • Department of Urology, The Johns Hopkins Medical Institutions, Baltimore, MD 21231, USA
    • Department of Oncology, The Johns Hopkins Medical Institutions, Baltimore, MD 21231, USA
    • ,
  • Syed Ali, MBBS

      Affiliations

    • Department of Pathology, The Johns Hopkins Medical Institutions, Baltimore, MD 21231, USA
    • ,
  • Angelo De Marzo, MD, PhD

      Affiliations

    • Department of Pathology, The Johns Hopkins Medical Institutions, Baltimore, MD 21231, USA
    • Department of Urology, The Johns Hopkins Medical Institutions, Baltimore, MD 21231, USA
    • Department of Oncology, The Johns Hopkins Medical Institutions, Baltimore, MD 21231, USA
    • ,
  • Alan K. Meeker, PhD

      Affiliations

    • Department of Pathology, The Johns Hopkins Medical Institutions, Baltimore, MD 21231, USA
    • Department of Urology, The Johns Hopkins Medical Institutions, Baltimore, MD 21231, USA
    • Corresponding Author InformationCorresponding author. Division of Genitourinary Pathology, Department of Pathology, The Johns Hopkins Medical Institutions, Baltimore, MD 21231, USA.

Received 10 November 2008 ,Revised 7 January 2009 ,Accepted 9 January 2009.

References 

  1. Bologna M, Vicentini C, Festuccia C, et al. Early diagnosis of prostatic carcinoma based on in vitro culture of viable tumor cells harvested by prostatic massage. Eur Urol. 1988;14:474–476
  2. Garret M, Jassie M. Cytologic examination of post prostatic massage specimens as an aid in diagnosis of carcinoma of the prostate. Acta Cytol. 1976;20:126–131
  3. Albers DD, Mc DJ, Thompson GJ. Carcinoma cells in prostatic secretions. J Am Med Assoc. 1949;139:299–303
  4. Guinan P, Gilham N, Nagubadi SR, Bush I, Rhee H, McKiel C. What is the best test to detect prostate cancer?. CA Cancer J Clin. 1981;31:141–145
  5. Sharifi R, Shaw M, Ray V, Rhee H, Nagubadi S, Guinan P. Evaluation of cytologic techniques for diagnosis of prostate cancer. Urology. 1983;21:417–420
  6. Koss LG, Deitch D, Ramanathan R, Sherman AB. Diagnostic value of cytology of voided urine. Acta Cytol. 1985;29:810–816
  7. Cairns P, Esteller M, Herman JG, et al. Molecular detection of prostate cancer in urine by GSTP1 hypermethylation. Clin Cancer Res. 2001;7:2727–2730
  8. Gonzalgo ML, Pavlovich CP, Lee SM, Nelson WG. Prostate cancer detection by GSTP1 methylation analysis of postbiopsy urine specimens. Clin Cancer Res. 2003;9:2673–2677
  9. Hessels D, Klein Gunnewiek JM, van Oort I, et al. DD3(PCA3)-based molecular urine analysis for the diagnosis of prostate cancer. Eur Urol. 2003;44:8–15[Discussion-6]
  10. Laxman B, Tomlins SA, Mehra R, et al. Noninvasive detection of TMPRSS2:ERG fusion transcripts in the urine of men with prostate cancer. Neoplasia. 2006;8:885–888
  11. Rogers CG, Yan G, Zha S, et al. Prostate cancer detection on urinalysis for alpha methylacyl coenzyme a racemase protein. J Urol. 2004;172:1501–1503
  12. Jeronimo C, Usadel H, Henrique R, et al. Quantitative GSTP1 hypermethylation in bodily fluids of patients with prostate cancer. Urology. 2002;60:1131–1135
  13. Tricoli JV, Schoenfeldt M, Conley BA. Detection of prostate cancer and predicting progression: current and future diagnostic markers. Clin Cancer Res. 2004;10:3943–3953
  14. Smith AM, Dave S, Nie S, True L, Gao X. Multicolor quantum dots for molecular diagnostics of cancer. Expert Rev Mol Diagn. 2006;6:231–244
  15. Bethel CR, Faith D, Li X, et al. Decreased NKX3.1 protein expression in focal prostatic atrophy, prostatic intraepithelial neoplasia, and adenocarcinoma: association with Gleason score and chromosome 8p deletion. Cancer Res. 2006;66:10683–10690
  16. Wu YL, Dudognon C, Nguyen E, et al. Immunodetection of human telomerase reverse-transcriptase (hTERT) re-appraised: nucleolin and telomerase cross paths. J Cell Sci. 2006;119:2797–2806
  17. Papanicolaou GN. A new procedure for staining vaginal smears. Science. 1942;95:438–439
  18. Djavan B, Zlotta A, Remzi M, et al. Optimal predictors of prostate cancer on repeat prostate biopsy: a prospective study of 1,051 men. J Urol. 2000;163:1144–1148[discussion 8-9]
  19. Keetch DW, Catalona WJ, Smith DS. Serial prostatic biopsies in men with persistently elevated serum prostate specific antigen values. J Urol. 1994;151:1571–1574
  20. Foot NC, Holmquist ND. Supravita staining of sediments of serous effusions; a simple technique for rapid cytological diagnosis. Cancer. 1958;11:151–157
  21. Garret M, Hamm FC. Atypical cells of origin from the seminal vesicles, complicating cytologic evaluation of prostatic secretions. Am J Clin Pathol. 1963;39:265–272
  22. De Marzo AM, DeWeese TL, Platz EA, et al. Pathological and molecular mechanisms of prostate carcinogenesis: implications for diagnosis, detection, prevention, and treatment. J Cell Biochem. 2004;91:459–477
  23. Luo J, Zha S, Gage WR, et al. Alpha-methylacyl-CoA racemase: a new molecular marker for prostate cancer. Cancer Res. 2002;62:2220–2226
  24. Meeker AK, Hicks JL, Platz EA, et al. Telomere shortening is an early somatic DNA alteration in human prostate tumorigenesis. Cancer Res. 2002;62:6405–6409
  25. Rubin MA, Zhou M, Dhanasekaran SM, et al. alpha-Methylacyl coenzyme A racemase as a tissue biomarker for prostate cancer. Jama. 2002;287:1662–1670
  26. Tomlins SA, Laxman B, Dhanasekaran SM, et al. Distinct classes of chromosomal rearrangements create oncogenic ETS gene fusions in prostate cancer. Nature. 2007;448:595–599
  27. Nilsson M. Lock and roll: single-molecule genotyping in situ using padlock probes and rolling-circle amplification. Histochem Cell Biol. 2006;126:159–164
  28. Speel EJ, Hopman AH, Komminoth P. Tyramide signal amplification for DNA and mRNA in situ hybridization. Methods Mol Biol. 2006;326:33–60
  29. Schmitt FC, Longatto-Filho A, Valent A, Vielh P. Molecular techniques in cytopathology practice. J Clin Pathol. 2008;61:258–267
  30. Ali TZ, Epstein JI, Bieberich CM, De Marzo AM. Nkx3.1 as a new tissue marker of prostatic adenocarcinoma. Mod Pathol. 2006;19:128A
  31. Kelemen PR, Buschmann RJ, Weisz-Carrington P. Nucleolar prominence as a diagnostic variable in prostatic carcinoma. Cancer. 1990;65:1017–1020
  32. Sturgis CD, Box M, D'Costa R, Forgue B, McGuire MS, Dieterich M. Ancillary alpha-methylacyl-CoA racemase immunocytochemistry in the diagnosis of adenocarcinoma of the prostate in urinary cytology: a case report. Acta Cytol. 2006;50:335–338
  33. Vener T, Derecho C, Baden J, et al. Development of a multiplexed urine assay for prostate cancer diagnosis. Clin Chem. 2008;54:874–882
  34. Kern WH, Bales CE, Webster WW. Cytologic evaluation of transitional cell carcinoma of the bladder. J Urol. 1968;100:616–622
  35. Ntouroupi TG, Ashraf SQ, McGregor SB, et al. Detection of circulating tumour cells in peripheral blood with an automated scanning fluorescence microscope. Br J Cancer. 2008;99:789–795

☆ This work was supported by the following research funding mechanisms: NIH/NIDDK grant 1K23DK071262 (C. P. Pavlovich), Department of Defense grant W81XWH-05-1-0167 (C. P. Pavlovich), and a Patrick C. Walsh Foundation grant (A. K. Meeker).

PII: S0046-8177(09)00011-2

doi: 10.1016/j.humpath.2009.01.004

Human Pathology
Volume 40, Issue 7 , Pages 924-933 , July 2009

Article Tools

* Image Usage & Resolution