Human Pathology
Volume 40, Issue 12 , Pages 1679-1685, December 2009

Down-regulation of ephrin-A5, a gene product of normal cartilage, in chondrosarcoma

  • Thomas Kalinski, MD

      Affiliations

    • Department of Pathology, Otto-von-Guericke-University, D-39120 Magdeburg, Germany
    • Corresponding Author InformationCorresponding author.
    • ,
  • Albrecht Röpke, PhD

      Affiliations

    • Department of Human Genetics, Westfalian Wilhelms-University, D-48149 Münster, Germany
    • ,
  • Saadettin Sel, MD

      Affiliations

    • Department of Ophthalmology, Martin-Luther-University, D-06120 Halle, Germany
    • ,
  • Irina Kouznetsova, PhD

      Affiliations

    • Department of Molecular Biology and Medical Chemistry, Otto-von-Guericke-University, D-39120 Magdeburg, Germany
    • ,
  • Martin Röpke, MD

      Affiliations

    • Department of Orthopedics, Otto-von-Guericke-University, D-39120 Magdeburg, Germany
    • ,
  • Albert Roessner, MD

      Affiliations

    • Department of Pathology, Otto-von-Guericke-University, D-39120 Magdeburg, Germany

Received 29 October 2008; received in revised form 24 February 2009; accepted 21 March 2009. published online 20 August 2009.

Summary 

As ephrins have been associated with tumorigenesis and tumor progression, we investigated ephrin-A5 (EFNA5) expression in specimens of normal cartilage and chondrosarcomas of different grade by conventional and quantitative reverse transcription polymerase chain reaction (RT-PCR), Western blot, and immunohistochemistry. We detected a significant EFNA5 down-regulation in chondrosarcomas compared with normal cartilage using quantitative RT-PCR (P < .05). The results were confirmed by Western blot and immunohistochemistry. We did not detect any causative genetic or epigenetic alterations in EFNA5 promoter methylation, loss of heterozygosity, or mutation analyses. Apart from slight differences in EFNA5 transcript amounts, we detected no significant influence of hypoxia on EFNA5 expression in C3842 and SW1353 chondrosarcoma cells. As EFNA5 down-regulation is a consistent finding in chondrosarcomas, we presume that it represents another essential alteration in tumorigenesis and tumor progression associated with cell adhesion, in addition to a multitude of other partially unknown biologic functions mediated by bidirectional ephrin/Eph receptor signaling and cross talk.

Keywords: Ephrin-A5, Chondrosarcoma, Cartilage tissue, Hypoxia

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PII: S0046-8177(09)00169-5

doi:10.1016/j.humpath.2009.03.024

Human Pathology
Volume 40, Issue 12 , Pages 1679-1685, December 2009

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