Human Pathology
Volume 41, Issue 2 , Pages 249-254 , February 2010

Imprint cytology detects floating Brachyspira in human intestinal spirochetosis

  • Sho Ogata, MD, PhD

      Affiliations

    • Division of Medicine, Japan Self Defense Forces Hospital Yokosuka, Yokosuka 237-0071, Japan
    • Department of Pathology and Laboratory Medicine, National Defense Medical College, Tokorozawa 359-8513, Japan
    • Corresponding Author InformationCorresponding author. Department of Pathology and Laboratory Medicine, National Defense Medical College, 3-2 Namiki, Tokorozawa, Saitama 359-8513, Japan.
    • ,
  • Masaaki Higashiyama, MD

      Affiliations

    • Division of Medicine, Japan Self Defense Forces Hospital Yokosuka, Yokosuka 237-0071, Japan
    • ,
  • Yoshikazu Adachi, DVM, PhD

      Affiliations

    • Department of Cell Physiology, School of Agriculture, Ibaraki University, Ibaraki 300-0393, Japan
    • ,
  • Ichiyo Ohara, MD, PhD

      Affiliations

    • Division of Medicine, Japan Self Defense Forces Hospital Yokosuka, Yokosuka 237-0071, Japan
    • ,
  • Junichiro Nishiyama, MD, PhD

      Affiliations

    • Division of Medicine, Japan Self Defense Forces Hospital Yokosuka, Yokosuka 237-0071, Japan
    • ,
  • Yasushi Okusa, MD, PhD

      Affiliations

    • Division of Medicine, Japan Self Defense Forces Hospital Yokosuka, Yokosuka 237-0071, Japan
    • ,
  • Hiroaki Takeo, MD, PhD

      Affiliations

    • Department of Pathology, Japan Self Defense Forces Central Hospital, Setagaya, Tokyo 154-8532, Japan
    • ,
  • Kimiya Sato, MD, PhD

      Affiliations

    • Department of Pathology, Japan Self Defense Forces Central Hospital, Setagaya, Tokyo 154-8532, Japan
    • ,
  • Kuniaki Nakanishi, MD, PhD

      Affiliations

    • Department of Pathology and Laboratory Medicine, National Defense Medical College, Tokorozawa 359-8513, Japan
    • ,
  • Toshiaki Kawai, MD, PhD

      Affiliations

    • Department of Pathology and Laboratory Medicine, National Defense Medical College, Tokorozawa 359-8513, Japan

Received 19 June 2009 ,Revised 22 July 2009 ,Accepted 30 July 2009.

References 

  1. Rotterdam H. Intestinal spirochetosis. In:  Connor DH,  Chandler FW,  Schwartz DA, et al. editor. Pathology of infectious diseases. Vol. 1:Stamford (Conn): Appleton & Lange; 1997;p. 583–588
  2. Korner M, Gebbers JO. Clinical significance of human intestinal spirochetosis—a morphological approach. Infection. 2003;31:341–349
  3. Peruzzi S, Gorrini C, Piccolo G, Calderaro A, Dettori G, Chezzi C. Human intestinal spirochaetosis in Parma: a focus on a selected population during 2002-2005. Acta Biomed. 2007;78:128–132
  4. Weisheit B, Bethke B, Stolte M. Human intestinal spirochetosis: analysis of the symptoms of 209 patients. Scand J Gastroenterol. 2007;42:1422–1427
  5. Willen R, Wanders A, Engstrand L. Intestinal spirochaetosis. Histopathology. 2006;49:658–659
  6. Abe Y, Hirane A, Yoshizawa A, Nakajima H, Adachi Y. The specific antibody to Brachyspira aalborgi in serum obtained from a patient with intestinal spirochetosis. J Vet Med Sci. 2006;68:1089–1091
  7. Higashiyama M, Ogata S, Adachi Y, et al. Human intestinal spirochetosis accompanied by human immunodeficiency virus infection: a case report. Acta Med Okayama. 2009;63:217–221
  8. Raber TL, Buckner L. Cytopathology techniques. In:  Mikel UV editors. Armed Forces Institute of Pathology Advanced Laboratory Methods in Histology and Pathology. Washington, DC.: American Registry of Pathology; 1994;p. 207–238
  9. Tasu C, Nakamura S, Tazawa H, Hara H, Adachi Y. Morphological properties of a human intestinal spirochetosis first isolated from a patient with diarrhea in Japan. Microbiol Immunol. 2003;47:989–996
  10. Deerinck TJ, Martone M, Ellisman MH. Preparative methods for transmission electron microscopy. In:  Spector DL,  Goldman RD editor. Basic methods in microscopy. protocols and concepts from cells: a laboratory manual. Cold Spring Harbor (NY): Cold Spring Harbor Laboratory Press; 2006;p. 307–341
  11. Kraaz W, Pettersson B, Thunberg U, Engstrand L, Fellstrom C. Brachyspira aalborgi infection diagnosed by culture and 16S ribosomal DNA sequencing using human colonic biopsy specimens. J Clin Microbiol. 2000;38:2555–2560
  12. Guccin JG, Benator DA, Zeller J, Termanini B, Saini N. Intestinal spirochetosis and acquired immunodeficiency syndrome: ultrastructural studies of two cases. Ultrastruct Pathol. 1995;19:15–22
  13. Nakamura S, Adachi Y, Goto T, Magariyama Y. Improvement in motion efficiency of the spirochete Brachyspira pilosicoli in viscous environments. Biophys J. 2006;90:3019–3026
  14. Meyer-Hoffert U, Hornef MW, Henriques-Normark B, et al. Secreted enteric antimicrobial activity localized to the mucus surface layer. Gut. 2008;57:764–771
  15. Jensen TK, Moller K, Boye M, Leser TD, Jorsal SE. Scanning electron microscopy and fluorescent in situ hybridization of experimental Brachyspira (Serpulina) pilosicoli infection in growing pigs. Vet Pathol. 2000;37:22–32
  16. Mikosza A, La T, Brooke CJ, et al. PCR amplification from fixed tissue indicated frequent involvement of Brachyspira aalborgi in human intestinal spirochetosis. J Clin Microbiol. 1999;37:2093–2098
  17. Mikosza A, La T, de Boer BW, Hampson DJ. Comparative prevalence of Brachyspira aalborgi and Brachyspira (Serpulina) pilosicoli as etiologic agents of histologically identified intestinal spirochetosis in Australia. J Clin Microbiol. 2001;39:347–350

☆ The contents of this article were presented at the 5th International Conference of Intestinal Spirochaetal Infections in Animals and Humans on June 8, 2009, in Leon, Spain.

☆☆ This work was supported in part by a grant from the Japan Ministry of Defense (to S. O.).

★ The views expressed in this article are those of the authors and do not reflect the official policies of the Japan Ministry of Defense or the Japan Self Defense Forces.

PII: S0046-8177(09)00282-2

doi: 10.1016/j.humpath.2009.07.020

Human Pathology
Volume 41, Issue 2 , Pages 249-254 , February 2010

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