p53 expression in tumor-stromal fibroblasts is closely associated with the nodal metastasis and outcome of patients with invasive ductal carcinoma who received neoadjuvant therapy

Hasebe, Takahiro; Tamura, Nobuko; Okada, Nao; Hojo, Takashi; Akashi-Tanaka, Sadako; Shimizu, Chikako; Tsuda, Histoshi; Shibata, Tatsuhiro; Sasajima, Yuko; Iwasaki, Motoki; Kinoshita, Takayuki

doi:10.1016/j.humpath.2009.07.021

« Previous Next »Human Pathology
Volume 41, Issue 2 , Pages 262-270, February 2010

p53 expression in tumor-stromal fibroblasts is closely associated with the nodal metastasis and outcome of patients with invasive ductal carcinoma who received neoadjuvant therapy☆

Takahiro Hasebe, MD, PhD
- Clinical Trials and Practice Support Division, Pathology Consultation Service, Center for Cancer Control and Information Services, National Cancer Center, Tokyo 104-0045, Japan
- Corresponding author.
Nobuko Tamura, MD
- Department of Breast Surgery, National Cancer Center Hospital, Tokyo 104-0045, Japan
Nao Okada, MD
- Department of Breast Surgery, National Cancer Center Hospital, Tokyo 104-0045, Japan
Takashi Hojo, MD
- Department of Breast Surgery, National Cancer Center Hospital, Tokyo 104-0045, Japan
Sadako Akashi-Tanaka, MD, PhD
- Department of Breast Surgery, National Cancer Center Hospital, Tokyo 104-0045, Japan
Chikako Shimizu, MD
- Division of Breast and Medical Oncology, National Cancer Center Hospital, Tokyo 104-0045, Japan
Histoshi Tsuda, MD, PhD
- Clinical Laboratory Division, National Cancer Center Hospital, Tokyo 104-0045, Japan
Tatsuhiro Shibata, MD, PhD
- Cancer Genomics Project, National Cancer Center Research Institute, Tokyo 104-0045, Japan
Yuko Sasajima, MD, PhD
- Clinical Laboratory Division, National Cancer Center Hospital, Tokyo 104-0045, Japan
Motoki Iwasaki, MD, PhD
- Epidemiology and Prevention Division, Research Center for Cancer Prevention and Screening, National Cancer Center, Tokyo 104-0045, Japan
Takayuki Kinoshita, MD, PhD
- Department of Breast Surgery, National Cancer Center Hospital, Tokyo 104-0045, Japan

Received 10 April 2009; received in revised form 27 July 2009; accepted 30 July 2009. published online 16 October 2009.

Summary

The purpose of this study was to determine whether p53 immunoreactivity in tumor-stromal fibroblasts assessed by the Allred scoring system in biopsy specimens obtained before neoadjuvant therapy and assessed in surgical specimens obtained after neoadjuvant therapy is significantly associated with nodal metastasis by invasive ductal carcinoma and with the outcome of 318 patients with invasive ductal carcinoma who received neoadjuvant therapy, according to UICC pathologic TNM stage, in multivariate analyses with well-known clinicopathologic factors. The Allred scores for p53 in tumor-stromal fibroblasts in the surgical specimens were significantly associated with the presence of nodal metastasis. The Allred scores for p53 in the tumor-stromal fibroblasts of biopsy and surgical specimens were a very important outcome predictive factor for patients who received neoadjuvant therapy, independent of UICC pathologic TNM status, but the outcome predictive power of the Allred scores for p53 in tumor-stromal fibroblasts assessed in the surgical specimens was superior to that of the Allred scores for p53 in tumor-stromal fibroblasts in the biopsy specimens. The results indicated a close association between p53 protein expression in tumor-stromal fibroblasts, especially in surgical specimens, and both the presence of nodal metastasis and the outcome of invasive ductal carcinoma patients who received neoadjuvant therapy.

Keywords: Breast cancer, p53, Fibroblast, Tumor stroma, Prognosis