Human Pathology
Volume 41, Issue 3 , Pages 336-342 , March 2010

Fluorescence in situ hybridization analysis of extraskeletal myxoid chondrosarcomas using EWSR1 and NR4A3 probes

  • Hiroko Noguchi, MD

      Affiliations

    • Department of Surgical Pathology, Sapporo Medical University School of Medicine, Sapporo 060-8543, Japan
    • ,
  • Tomoko Mitsuhashi, MD, PhD

      Affiliations

    • Department of Surgical Pathology, Sapporo Medical University School of Medicine, Sapporo 060-8543, Japan
    • ,
  • Kunihiko Seki, MD, PhD

      Affiliations

    • Division of Clinical Laboratory, JR Tokyo General Hospital, Tokyo 151-8528, Japan
    • ,
  • Naofumi Tochigi, MD, PhD

      Affiliations

    • Division of Pathology, National Cancer Center Research Institute, Tokyo 104-0045, Japan
    • ,
  • Masahisa Tsuji, PhD

      Affiliations

    • Chromosome Science Lab Inc., Sapporo 003-0801, Japan
    • ,
  • Tadakazu Shimoda, MD, PhD

      Affiliations

    • Division of Clinical Laboratory, National Cancer Center Hospital, Tokyo 104-0045, Japan
    • ,
  • Tadashi Hasegawa, MD, PhD

      Affiliations

    • Department of Surgical Pathology, Sapporo Medical University School of Medicine, Sapporo 060-8543, Japan
    • Corresponding Author InformationCorresponding author.

Received 2 February 2009 ,Revised 30 March 2009 ,Accepted 2 April 2009.

References 

  1. Meis-Kindblom JM, Bergh P, Gunterberg B, Kindblom LG. Extraskeletal myxoid chondrosarcoma: a reappraisal of its morphologic spectrum and prognostic factors based on 117 cases. Am J Surg Pathol. 1999;23:636–650
  2. Kawaguchi S, Wada T, Nagoya S, et al. Extraskeletal myxoid chondrosarcoma: a multi- institutional study of 42 cases in Japan. Cancer. 2003;97:1285–1292
  3. Drilon AD, Popat S, Bhuchar G, et al. Extraskeletal myxoid chondrosarcoma: a retrospective review from 2 referral centers emphasizing long-term outcomes with surgery and chemotherapy. Cancer. 2008;113:3364–3371
  4. Hisaoka M, Hashimoto H. Extraskeletal myxoid chondrosarcoma: updated clinicopathological and molecular genetic characteristics. Pathol Int. 2005;55:453–463
  5. Sjögren H, Meis-Kindblom J, Kindblom LG, Aman P, Stenman G. Fusion of the EWS- related gene TAF2N to TEC in extraskeletal myxoid chondrosarcoma. Cancer Res. 1999;59:5064–5067
  6. Bjerkehagen B, Dietrich C, Reed W, et al. Extraskeletal myxoid chondrosarcoma: multimodal diagnosis and identification of a new cytogenetic subgroup characterized by t(9;17)(q22;q11). Virchows Arch. 1999;435:524–530
  7. Panagopoulos I, Mertens F, Isaksson M, et al. Molecular genetic characterization of the EWS/CHN and RBP56/CHN fusion genes in extraskeletal myxoid chondrosarcoma. Genes Chromosomes Cancer. 2002;35:340–352
  8. Sjögren H, Wedell B, Meis-Kindblom JM, Kindblom LG, Stenman G. Fusion of the NH2-terminal domain of the basic helix-loop-helix protein TCF12 to TEC in extraskeletal myxoid chondrosarcoma with translocation t(9;15)(q22;q21). Cancer Res. 2000;60:6832–6835
  9. Hisaoka M, Ishida T, Imamura T. Hashimoto H. TFG is a novel fusion partner of NOR1 in extraskeletal myxoid chondrosarcoma. Genes Chromosomes Cancer. 2004;40:325–328
  10. Wang WL, Mayordomo E, Czerniak BA, et al. Fluorescence in situ hybridization is a useful ancillary diagnostic tool for extraskeletal myxoid chondrosarcoma. Mod Pathol. 2008;21:1303–1310
  11. Tateishi U, Hasegawa T, Nojima T, Takegami T, Arai Y. MRI features of extraskeletal myxoid chondrosarcoma. Skeletal Radiol. 2006;35:27–33
  12. Okamoto S, Hisaoka M, Ishida T, et al. Extraskeletal myxoid chondrosarcoma: a clinicopathologic, immunohistochemical, and molecular analysis of 18 cases. Hum Pathol. 2001;32:1116–1124
  13. Sjögren H, Meis-Kindblom JM, Orndal C, et al. Studies on the molecular pathogenesis of extraskeletal myxoid chondrosarcoma-cytogenetic, molecular genetic, and cDNA microarray analyses. Am J Pathol. 2003;162:781–792
  14. Yamaguchi U, Hasegawa T, Morimoto Y, et al. A practical approach to the clinical diagnosis of Ewing's sarcoma/primitive neuroectodermal tumour and other small round cell tumours sharing EWS rearrangement using new fluorescence in situ hybridisation probes for EWSR1 on formalin fixed, paraffin wax embedded tissue. J Clin Pathol. 2005;58:1051–1056
  15. Matsumura T, Yamaguchi T, Seki K, et al. Advantage of FISH analysis using FKHR probes for an adjunct to diagnosis of rhabdomyosarcomas. Virchows Arch. 2008;452:251–258
  16. Sugita S, Seki K, Yokozawa K, et al. Analysis of CHOP rearrangement in pleomorphic liposarcomas using fluorescence in situ hybridization. Cancer Sci. 2009;100:82–87
  17. Downs-Kelly E, Goldblum JR, Patel RM, et al. The utility of fluorescence in situ hybridization (FISH) in the diagnosis of myxoid soft tissue neoplasms. Am J Surg Pathol. 2008;32:8–13
  18. Kohashi K, Oda Y, Yamamoto H, et al. SMARCB1/INI1 protein expression in round cell soft tissue sarcomas associated with chromosomal translocations involving EWS: a special reference to SMARCB1/INI1 negative variant extraskeletal myxoid chondrosarcoma. Am J Surg Pathol. 2008;32:1168–1174

PII: S0046-8177(09)00303-7

doi: 10.1016/j.humpath.2009.04.028

Human Pathology
Volume 41, Issue 3 , Pages 336-342 , March 2010

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