Human Pathology
Volume 41, Issue 4 , Pages 610-613 , April 2010

Mixed infection of Trypanosoma cruzi I and II in a Colombian cardiomyopathic patient

  • Julio C. Mantilla, MD

      Affiliations

    • Grupo de Inmunología y Epidemiología Molecular, GIEM, Facultad de Salud, Universidad Industrial de Santander, Colombia AA678
    • Departamento de Patología, Escuela de Medicina, Facultad de Salud, Universidad Industrial de Santander, Colombia
    • ,
  • German A. Zafra, MSc

      Affiliations

    • Grupo de Inmunología y Epidemiología Molecular, GIEM, Facultad de Salud, Universidad Industrial de Santander, Colombia AA678
    • ,
  • Andrea M. Macedo, PhD

      Affiliations

    • Departamento de Bioquímica e Imunología, Universidade Federal de Minas Gerais, Brasil
    • ,
  • Clara Isabel González, PhD

      Affiliations

    • Grupo de Inmunología y Epidemiología Molecular, GIEM, Facultad de Salud, Universidad Industrial de Santander, Colombia AA678
    • Corresponding Author InformationCorresponding author. Escuela de Bacteriología, Universidad Industrial de Santander, Bucaramanga, Colombia.

Received 17 August 2009 ,Revised 3 November 2009 ,Accepted 4 November 2009.

References 

  1. World Health Organization . Control of Chagas disease. Second report of the WHO expert committee. World Health Organ Tech Rep Ser. 2002;905:1–109
  2. Tibayrenc M. Population genetics of parasitic protozoa and other microorganisms. Adv Parasitol. 1995;36:47–115
  3. Souto RP, Fernandes O, Macedo AM, Campbell DA, Zingales B. DNA markers define two major phylogenetic lineages of Trypanosoma cruzi. Mol Biochem Parasitol. 1996;83:141–152
  4. Brisse S, Verhoef J, Tibayrenc M. Characterization of large and small subunit rRNA and mini-exon genes further supports the distinction of six Trypanosoma cruzi lineages. Int J Parasitol. 2001;31:1218–1226
  5. Anonymous . Recommendations from a satellite meeting. Mem Inst Oswaldo Cruz. 1999;94:429–432
  6. Macedo AM, Machado CR, Oliveira RP. Pena SD. Trypanosoma cruzi: genetic structure of populations and relevance of genetic variability to the pathogenesis of Chagas disease. Mem Inst Oswaldo Cruz. 2004;99:1–12
  7. Montilla MM, Guhl F, Jaramillo C, et al. Isoenzyme Clustering of Trypanosomatidae Colombian Populations. Am J Trop Med Hyg. 2002;66:394–400
  8. Higo H, Miura S, Horio M, et al. Genotypic variation among lineages of Trypanosoma cruzi and its geographic aspects. Parasitol Int. 2004;53:337–344
  9. Añez N, Crisante G, da Silva FM, et al. Predominance of lineage I among Trypanosoma cruzi isolates from Venezuelan patients with different clinical profiles of acute Chagas' disease. Trop Med Int Health. 2004;9:1319–1326
  10. Miller SA, Dykes DD, Polesky HF. A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Res. 1988;16:1215
  11. Avila HA, Pereira JB, Thiemann O, et al. Detection of Trypanosoma cruzi in blood specimens of chronic chagasic patients by polymerase chain reaction amplification of kinetoplast minicircle DNA: comparison with serology and xenodiagnosis. J Clin Microbiol. 1993;31:2421–2426
  12. Moser DR, Kirchhoff L, Donelson JE. Detection of Trypanosoma cruzi by DNA amplification using the polymerase chain reaction. J Clin Microbiol. 1989;27:1477–1482
  13. Freitas JM, Augusto-Pinto L, Pimenta J, et al. Ancestral Genomes, Sex, and the Population Structure of Trypanosoma cruzi. PLoS Pathogens. 2006;2:e24
  14. Salazar A, Schijman AG, Triana-Chávez O. High variability of Colombian Trypanosoma cruzi lineage I stocks as revealed by low-stringency single primer-PCR minicircle signatures. Acta Trop. 2006;100:110–118
  15. Zafra G, Mantilla JC, Valadares HM, Macedo AM, González CI. Evidence of Trypanosoma cruzi II infection in Colombian chagasic patients. Parasitol Res. 2008;103:731–734

☆ This work was supported by Colciencias, grant 1102-05-17591, and Universidad Industrial de Santander, Colombia.

PII: S0046-8177(09)00408-0

doi: 10.1016/j.humpath.2009.11.005

Human Pathology
Volume 41, Issue 4 , Pages 610-613 , April 2010

Article Tools

* Image Usage & Resolution